Fig. 3

Effects of JS-K on apoptosis in SMMC7721 and HepG2 cells. a Western blotting analysis of Bcl-2, Bax, and Cyt c in mitochondria and cytosol of cells. b Western blotting analysis of caspase-9/− 3, cleaved caspase-9/− 3, PARP, cleaved PARP and XIAP of cells. c Effect of caspase inhibitors on JS-K-induced apoptosis. The cells were pretreated with caspase inhibitor Z-VAD-FMK (50 μM) for 1 h before treatment with 10 μM JS-K for 24 h and protein expression was assessed by Western blotting analysis. Data are mean ± SD. n = 3 for each concentration. *P < 0.05,**P < 0.01, vs. control group, ^#P < 0.05, ^##P < 0.01 vs. cells treated with JS-K alone