Fig. 4

NO was involved in the apoptosis induced by JS-K. a The levels of NO were determined using DAF-FM DA staining by fluorescence microscope. b The levels of NO were determined using DAF-FM DA staining by flow cytometry. c Effects of Carboxy-PTIO on the apoptosis in JS-K-treated cells. The cells were treated with the NO scavenger Carboxy-PTIO (50 μM) before treatment with 10 μM JS-K for 24 h and the apoptosis was assessed by flow cytometry. d Effects of Carboxy-PTIO on the apoptotic-related protein in JS-K-treated cells. The cells were treated with the NO scavenger Carboxy-PTIO (50 μM) before treatment with 10 μM JS-K for 24 h and the protein expressions of Bcl-2, Bax, cleaved-caspase-9/3 and cleaved-PARP were assessed by Western blotting analysis. Data are mean ± SD. n = 3 for each concentration. *P < 0.05,**P < 0.01, vs. control group, ^#P < 0.05, ^##P < 0.01 vs. cells treated with JS-K alone