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Fig. 5 | Journal of Experimental & Clinical Cancer Research

Fig. 5

From: Protein phosphatase 2A activation mechanism contributes to JS-K induced caspase-dependent apoptosis in human hepatocellular carcinoma cells

Fig. 5

JS-K increased the activity of PP2A in SMMC7721 and HepG2 cells. a The levels of PP2A were measured by ELISA kit. The cells were treated with different concentrations of JS-K for 24 h and the cell lysates were prepared and assayed by enzyme-linked immunosorbent assay. b The effects of JS-K on protein levels of PP2A complexs. c Effects of JS-K on protein levels of PP2A substrates. d Effects of Carboxy-PTIO on the expression of PP2A-C and PP2A substrates. e Effects of JS-K on protein levels of PP2A substrates through silencing PP2A-C. f Effects of JS-K on protein levels of PP2A substrates through overexpression of PP2A-C. The cells were transfected for 48 h before treatment with JS-K for 24 h. Data are mean ± SD. n = 3 for each concentration. *P < 0.05, **P < 0.01, vs. cells untreated with JS-K of control siRNA /negative plasmid group, #P < 0.05, ##P < 0.01 vs cells treated with JS-K of control siRNA /negative plasmid group, P < 0.05, P < 0.01, vs cells untreated with JS-K of PP2A siRNA/PP2A cDNA group

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