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Fig. 4 | Journal of Experimental & Clinical Cancer Research

Fig. 4

From: miR-182 suppresses invadopodia formation and metastasis in non-small cell lung cancer by targeting cortactin gene

Fig. 4

Cortactin was a direct target for miR-182 in NSCLC cells. The expression levels of cortactin mRNA and protein were down-regulated when transfected with miR-182 mimics in A549 and H1299 cells, while cortactin mRNA and protein expression in 95C cells were up-regulated when treated with miR-182 inhibitor; these were shown using (a) real-time PCR assays and (b)Western blotting, respectively. *P < 0.05 and **P < 0.01. c Predicted miR-182 target sequence in the 3’-UTR of CTTN mRNA. The mutant binding site is underlined. d Luciferase reporter assay of HEK-293 cells co-transfected with wild-type or mutant cortactin3’-UTR reporter plasmid and miR-182 or scrambled miRNA. *P < 0.05 (e) The level of miR-182 was inversely correlated with cortactin expression in human NSCLC tissues. Cortactin and miR-182 expression levels were detected by qRT-PCR

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