Fig. 5

miR-182 suppressed PDBu- and HGF-induced invadopodia formation and metastasis of lung cancer cells by regulating cortactin. a Transwell analysis of migrated and invaded A549 cells treated with miR-182 or scrambled miRNA. Representative images are displayed. Scale bar, 200 μm, 20× magnification, **P < 0.01. b and c A549 and H1299 cells were transfected with miR-182 or scrambled miRNA and then subjected to transwell assay in the presence of HGF treatment for 6 h or PDBu treatment for 30 min. Representative figures of the migrated and invaded stained cells are shown on the left. Data are shown as the mean ± SD. Scale bar, 200 μm, 20× magnification, *P < 0.05. d and e A549 and H1299 cells were transfected with scrambled miRNA, miR-182, si-NC or CTTN siRNA in the presence of (d) PDBu treatment for 30 min or (E) HGF treatment for 6 h, and stained for cortactin (green), F-actin (red), and nuclei (blue). Cells with invadopodia were quantified, and representative images are displayed on the right. Data are shown as the mean ± SD. Scale bar, 50 μm, 40× magnification, *P < 0.05. f After treatment with PDBu or HGF, protein expression of CTTN in A549 and H1299 cells transfected with scrambled miRNA or miR-182 were analyzed by SDS-PAGE. β-actin was used as a control