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Fig. 3 | Journal of Experimental & Clinical Cancer Research

Fig. 3

From: High expression of Ras-related protein 1A promotes an aggressive phenotype in colorectal cancer via PTEN/FOXO3/CCND1 pathway

Fig. 3

Knockdown of RAP1A inhibits the growth of CRC cells in vitro. a: qRT-PCR detected the mRNA level of RAP1A gene is higher in CRC cell lines as compared with normal intestinal epithelial cells. b: qRT-PCR and western blot showed both mRNA and protein expression of RAP1A is lower in knockdown (KD) group than that of negative control (NC) group. c: the relative 490 nm absorbance of KD group increases more slowly than that of NC group within five days. d: upper: there is no significant difference in cycle progression after RAP1A knockdown in HT-29 cells; lower: RAP1A knockdown decreases G1 phase fraction but increases G2/M phase fraction in SW620 cells. e: the apoptosis rates of KD group are higher than that of NC group. f: the clone numbers of KD group are less than that of NC group. ** means p < 0.01

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