Skip to main content
Fig. 3 | Journal of Experimental & Clinical Cancer Research

Fig. 3

From: BMP4 promotes hepatocellular carcinoma proliferation by autophagy activation through JNK1-mediated Bcl-2 phosphorylation

Fig. 3

BMP4 activated-autophagy promoted HCC cells proliferation in vitro: a & b HepG2 and HCCLM3 were treated with autophagy inhibitor 3-MA, BMP4 + 3-MA and BMP4 alone. CCK-8 assays were applied to detect cell viability in HCC cells with different treatment. n = 3, one-way ANOVA with post-hoc Tukey’s test. c & d Effects of autophagy inhibitor 3-MA on BMP4 promoted-colony formation ability in HCC cells. Combination of 3-MA and BMP4 significantly decreased the number of colonies as compared to the BMP4-treated groups. n = 3, one-way ANOVA with post-hoc Tukey’s test. e Transfection efficiency of siRNA targeting BECN1 in HCC cells was confirmed by Western blot. f The expression level of LC3-II was detected by Western blot. Knockdown of BECN1 decreased the expression of LC3-II as compared with the control groups and prevented autophagy activated by BMP4. g & h Knockdown of BECN1 significantly attenuated cell viability promoted by BMP4 both in HepG2 and HCCLM3 cells. Cell viability was determined by CCK-8. n = 3, one-way ANOVA with post-hoc Tukey’s test

Back to article page