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Fig. 6 | Journal of Experimental & Clinical Cancer Research

Fig. 6

From: BMP4 promotes hepatocellular carcinoma proliferation by autophagy activation through JNK1-mediated Bcl-2 phosphorylation

Fig. 6

Knockdown of JNK1 attenuated BMP4-activated autophagy and HCC proliferation: a Transfection efficiency of siRNA targeting JNK1 in HCC cells was confirmed by Western blot. b The expression level of LC3-II, p62 and BECN1 were detected by Western blot. Knockdown of JNK1 decreased BMP4-promoted LC3-II conversion as compared with the BMP4-treated group. c The expression levels of LC3-II were quantified by Image lab software by densitometric analysis and were normalized to the control groups. GAPDH was used as the internal control. n = 3, one-way ANOVA with post-hoc Tukey’s test. d Transfected cells were treated with BMP4 for 24 h. Cell viability was determined by CCK-8. n = 3, one-way ANOVA with post-hoc Tukey’s test

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