Skip to main content
Fig. 3 | Journal of Experimental & Clinical Cancer Research

Fig. 3

From: Osteopontin alters DNA methylation through up-regulating DNMT1 and sensitizes CD133+/CD44+ cancer stem cells to 5 azacytidine in hepatocellular carcinoma

Fig. 3

OPN alters DNA methylation in CD133+/CD44+ cells. a The ratio of mC in total cytosine in CD133+/CD44+ cells with SCR or shOPN from Huh7 and Hep3B, *, p < 0.05. b Dot blot of DNA isolated from CD133+/CD44+ cells with SCR or shOPN from Huh7 and Hep3B probed with anti-5mC antibody. Quantified signal was normalized to total DNA as measured by methylene blue staining, *, p < 0.05. c IHC staining for 5mC and Ki67 in the tumor tissues formed by CD133+/CD44+ cells with SCR or shOPN from Huh7. d MeDIP-seq results of RASSF1, CDKL2 and GATA4 genes (up) and confirmation by MSP-PCR (low)

Back to article page

Journal of Experimental & Clinical Cancer Research

ISSN: 1756-9966

Contact us