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Fig. 4 | Journal of Experimental & Clinical Cancer Research

Fig. 4

From: Osteopontin alters DNA methylation through up-regulating DNMT1 and sensitizes CD133+/CD44+ cancer stem cells to 5 azacytidine in hepatocellular carcinoma

Fig. 4

DNMT1 mediates OPN altering DNA methylation in CD133+/CD44+ cells. a Compared the protein file of CD133+/CD44+ SCR with that of CD133+/CD44+ shOPN sorted from Huh7, the top different proteins were picked up. b Using IHC and western blot, DNMT1 was down-regulated when knocking down OPN. c The ratio of mC in total cytosine in CD133+/CD44+ cells with SCR or shOPN/EV or shOPN/DNMT1 from Huh7 and Hep3B, *, p < 0.05, ns, no significance. d Dot blot of DNA isolated from CD133+/CD44+ cells with SCR or shOPN/EV or shOPN/DNMT1 from Huh7 and Hep3B probed with anti-5mC antibody. e Quantified signal was normalized to total DNA as measured by methylene blue staining, ns, no significance. f IHC analysis of histone 3 methyl marks, H3K4me3, H3K9me3 and H3K27me3 in the tumor tissues formed by CD133+/CD44+ cells with SCR or shOPN from Huh7

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