Fig. 8

HCCs with high OPN are more sensitive to DNMT1 inhibitor, 5 Aza, in vivo. a Volume of subcutaneous tumors derived from Huh7 cells and CD133+/CD44+ cells with EV or OPN isolated from Huh7 treated with PBS or 5 Aza (left), and body weight of mice with subcutaneous implantation of Huh7 cells and CD133+/CD44+ cells with EV or OPN (right). b Two HCC primary xenograft models treated with PBS or 5 Aza. Tumor size and volume between the PBS-treated and 5 Aza-treated tumors, **, p < 0.01, ***, p < 0.001. c-d A mean tumor volume ratio (PBS:5 Aza) and a mean tumor mass ratio (PBS:5 Aza) were seen in PDTX, *, p < 0.05, ***, p < 0.001. (E) IHC staining of DNMT1 and Ki67 in the subcutaneous tumor tissues from HCC primary xenograft models treated with PBS or 5 Aza