Fig. 2
DLD-1R cells exhibits increased tumor initiating ability in vivo. a Comparative in vivo tumor initiating ability between DLD-1R versus naïve DLD-1 counterparts. NOD/SCID mice were injected with the same number of DLD-1R and naïve cells (1 × 106 cells/injection) and tumor growth was monitored over time. DLD-1R bearing mice showed a significantly higher tumor burden as compared to their naïve counterparts. Photographs of harvested tumor samples are shown. b Representative FACS analysis demonstrated the Increased percentage of SP cells in tumor harvested from DLD-1R mice comparing to DLD-1 naïve samples. c Comparative immunohistochemical analysis showing an elevated expression of WNT1, NF-kB, Notch-1 and decreased expression of E-cadherin. The inserts show the magnified view of the corresponding sections