Fig. 5

Inhibitory effect of regorafenib and/or 5-FU on tumor sphere generation of DLD-1R and HCT-116R cells. Colonospheres were treated for three or seven days with complete CSCs medium containing DMSO (control), or 10 μM rogozafenib, or 10 μM 5-FU, alone or in combination. a Representative cell micrographs after three or seven days of treatment are shown. The area of the culture plates occupied by the spheres (b) were scored after three or seven days of treatment. The counts represent the mean values SEM of three independent experiments. The significance of differences was determined with a one-way ANOVA with Bonferroni post-test: ** p,0.05, *** p,0.001 vs. control