Fig. 1
Oxaliplatin suppresses the growth of HCT116 p53-wild-type cells by inducing apoptosis, but no such effect is seen in p53-null cells. A-C, HCT116 cells were treated without (a) or with (b and c) oxaliplatin and cell growth was dynamically monitored using impedance technology. Normalized cell index values measured over 70 h are shown. d, HCT116 cells were treated with oxaliplatin or ddH2O for different concentrations or time length and the percentage of apoptotic cells was determined by flow cytometry. The results are presented as the percentage of apoptotic cells; the presented values (mean ± SEs) represent three independent experiments performed in at least triplicate (*p < 0.05, ***p < 0.001 for cells treated with oxaliplatin vs. controls). Representative images are shown. e, HCT116 cells were treated with oxaliplatin or ddH2O for 24 h, and cell lysates were separated by SDS-PAGE and analyzed by Western blotting. β-Actin was used as an internal control. Representative images are shown