Fig. 5

Oxaliplatin decreases tNOX expression in HCT116 p53-wild-type cells but not in p53-null cells. Cells were pretreated with 50 μg/mL CHX (a) or MG132 (b, c) for 30 min and exposed to oxaliplatin (10 μM) for 24 h, and then analyzed for tNOX expression. The protein intensity of tNOX was determined by densitometric analysis and normalized to the relevant β-actin value. The presented results represent at least three independent experiments (*p < 0.05 or ***p < 0.001 for treatments vs. controls)