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Fig. 4 | Journal of Experimental & Clinical Cancer Research

Fig. 4

From: circFBLIM1 act as a ceRNA to promote hepatocellular cancer progression by sponging miR-346

Fig. 4

circFBLIM1 and FBLIM1 act as ceRNAs in HCC through regulation of miR-346. a The expression level of FBLIM1 was determined by qRT-PCR (left) and Western blot (right) in HCC cell lines. β-actin was used as a control. b The expression level of FBLIM1 in 50 HCC tissues and matched para-carcinoma normal tissues was determined by qRT-PCR. c The predicted binding sites of miR-346 within FBLIM1 were shown. d Luciferase assay of cells co-transfected with miR-346 mimics or miR-346 LNA and luciferase reporter containing FBLIM1 3′-UTR (FBLIM1 wt) or mutant construct (FBLIM1 mut). e Cells were transfected as described, and the expression of FBLIM1 was determined by qRT-PCR. f Cells were transfected with si-NC or si-FBLIM1, and the expression of miR-346 was determined by qRT-PCR. g Cells were transfected with NC, si-FBLIM1 or si-FBLIM1 + miR-346 LNA, and the expression of circFBLIM1 was determined by qRT-PCR. h Cells were transfected with NC, si-circFBLIM1 or si-circFBLIM1 + miR-346 LNA, and the expression of FBLIM1 was determined by qRT-PCR. i Cells were transfected with miR-NC, miR-346 or miR-346 + si-circFBLIM1, and CCK8 assay was performed. j Cells were transfected as described, and apoptosis assay was performed. Original magnification, × 400. All the data are shown as the mean ± S.D., *P < 0.05 and **P < 0.01

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