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Fig. 1 | Journal of Experimental & Clinical Cancer Research

Fig. 1

From: PCBP1 depletion promotes tumorigenesis through attenuation of p27Kip1 mRNA stability and translation

Fig. 1

PCBP1 binds to p27 transcript to increase its expression. (a) Identification of the purely PCBP1-bound mRNAs by RNA sequencing. mRNA numbers are indicated as copy number. (b) RT-PCR validation of PCBP1-associated p27 mRNA from the precipitated mRNA pools in A. p21 and GAPDH transcripts are respectively used as the positive and negative controls of PCBP1-bound mRNA. (c) RT-PCR detection of endogenous p27 mRNA immunoprecipitated by PCBP1-specific antibody. Normal IgG is used as negative control for RIP. c-Myc and GAPDH are used as positive or negative control of PCBP1-bound mRNA, respectively. (d) Quantitative RT-PCR detection of p27 mRNA in A2780 cells with overexpressing or silencing endogenous PCBP1. Three independent experiments were carried out and analyzed. Data are shown as means±SD. *p<0.05; ***p<0.001. (e) Immunoblot of p27 protein expression in the indicated cells with PCBP1 overexpression or silence by its specific shRNAs. A positive correlation between PCBP1 and p27 protein level is observed in these cell lines

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