Fig. 1

PBX3 is upregulated in mesenchymal gliomas and promotes glioma mesenchymal transition. a-c PBX3 expression is significantly higher in mesenchymal gliomas than that in proneural gliomas in Rembrandt (a), GSE4290 (b) and GSE59612 (c) datasets. d GSEA plot showed that hallmark of epithelial mesenchymal transition was significantly enriched in high PBX3 expression samples of GBM. e Verification of PBX3 overexpression by western blot assay. f The morphology of U87 and U251 cells infected with LV-NC or LV-PBX3 were compared under bright-field. Red arrows showed the morphological changes of U87 and U251 cells upon PBX3 overexpression. Scale bar = 100 μm. g Western blot analysis of N-cadherin, ZEB1, Slug and CD44 expressions upon PBX3 overexpression. h Verification of PBX3 knockdown by western blot assay. i The morphology of U87 and U251 cells infected with LV-siRNA-NC or LV-siRNA-PBX3 were compared under bright-field. Yellow arrows showed the morphological changes of U87 and U251 cells upon PBX3 knockdown. Scale bar = 100 μm. j Western blot analysis of N-cadherin, ZEB1, Slug and CD44 upon PBX3 knockdown