Fig. 3
PBX3 promotes GBM mesenchymal transition, migration and invasion is mediated by LIN28/let-7b axis. a PBX3 overexpression remarkably upregulated LIN28 protein levels and downregulated let-7b expression in U87 cells. b U87 cells stably expressing LV-PBX3 or LV-NC were transfected with siRNA-NC or siRNA-LIN28 and then immunoblotting analysis of LIN28, N-cadherin, ZEB1, Slug and CD44 were performed. c Quantification of wound-healing (left) and transwell (right) assays. **indicates a statistical significant difference (p < 0.01) between LV-NC + siRNA-NC group and LV-NC + siRNA-LIN28 group. # indicates a statistical significant difference (p < 0.01) between LV-NC + siRNA-NC group and LV-PBX3 + siRNA-NC group. $ indicates a statistical significant difference (p < 0.01) between LV-PBX3 + siRNA-NC group and LV-PBX3 + siRNA-LIN28 group. d U87 cells stably expressing LV-PBX3 or LV-NC were transfected with pre-miR-NC or pre-let-7b and then let-7b expression were determined by qRT-PCR. **indicates a statistical significant difference (p < 0.01) between LV-NC + pre-miR-NC group and LV-NC + pre-let-7b group. # indicates a statistical significant difference (p < 0.01) between LV-NC + pre-miR-NC group and LV-PBX3 + pre-miR-NC group. $ indicates a statistical significant difference (p < 0.01) between LV-PBX3 + pre-miR-NC group and LV-PBX3 + pre-let-7b group. e U87 cells stably expressing LV-PBX3 or LV-NC were transfected with pre-miR-NC or pre-let-7b and then immunoblotting analysis of N-cadherin, ZEB1, Slug and CD44 were performed. f Quantification of wound-healing (left) and transwell (right) assays. **indicates a statistical significant difference (p < 0.01) between LV-NC + pre-miR-NC group and LV-NC + pre-let-7b group. # indicates a statistical significant difference (p < 0.01) between LV-NC + pre-miR-NC group and LV-PBX3 + pre-miR-NC group. $ indicates a statistical significant difference (p < 0.01) between LV-PBX3 + pre-miR-NC group and LV-PBX3 + pre-let-7b group. g PBX3 knockdown remarkably downregulated LIN28 protein levels and upregulated let-7b expression in U87 cells. h U87 cells stably expressing LV-siRNA-PBX3 or LV-siRNA-NC were transfected with LIN28 overexpressing plasmids or empty vectors and then immunoblotting analysis of LIN28, N-cadherin, ZEB1, Slug and CD44 were performed. i Quantification of wound-healing (left) and transwell (right) assays. **indicates a statistical significant difference (p < 0.01) between LV-siRNA-NC + vector group and LV-siRNA-NC + LIN28 group. # indicates a statistical significant difference (p < 0.01) between LV-siRNA-NC + vector group and LV-siRNA-PBX3 + vector group. $ indicates a statistical significant difference (p < 0.01) between LV-siRNA-PBX3 + vector group and LV-siRNA-PBX3 + LIN28 group. j U87 cells stably expressing LV-siRNA-PBX3 or LV-siRNA-NC were transfected with anti-miR-NC or anti-let-7b and then let-7b expression were determined by qRT-PCR. **indicates a statistical significant difference (p < 0.01) between LV-siRNA-NC + anti-miR-NC group and LV-siRNA-NC + anti-let-7b group. # indicates a statistical significant difference (p < 0.01) between LV-siRNA-NC + anti-miR-NC group and LV-siRNA-PBX3 + anti-miR-NC group. $ indicates a statistical significant difference (p < 0.01) between LV-siRNA-PBX3 + anti-miR-NC group and LV-siRNA-PBX3 + anti-let-7b group. k U87 cells stably expressing LV-siRNA-PBX3 or LV-siRNA-NC were transfected with anti-miR-NC or anti-let-7b and then immunoblotting analysis of N-cadherin, ZEB1, Slug and CD44 were performed. l Quantification of wound-healing (left) and transwell (right) assays. **indicates a statistical significant difference (p < 0.01) between LV-siRNA-NC + anti-miR-NC group and LV-siRNA-NC + anti-let-7b group. # indicates a statistical significant difference (p < 0.01) between LV-siRNA-NC + anti-miR-NC group and LV-siRNA-PBX3 + anti-miR-NC group. $ indicates a statistical significant difference (p < 0.01) between LV-siRNA-PBX3 + anti-miR-NC group and LV-siRNA-PBX3 + anti-let-7b group