Fig. 2

ER stress induced KLF4 expression. a-d Mel-RM cells were treated with 3 μM TM at the indicated times. Gene expression profiles were obtained by mRNA microarray analysis. e-f The expression levels of the KLF family genes KLF4, KLF5, KLF6 and KLF8 were analysed in Mel-RM cells with or without TM treatment by q-RT-PCR and western blot. g-h Me1007 cells were treated with 3 μM TM at the indicated times. The expression levels of the KLF family genes KLF4, KLF5, KLF6 and KLF8 were detected by q-RT-PCR and western blot. i The protein levels of KLF4 were analysed in Me1007, Mel-CV, SK-Mel-28, A375 and Mel-RM cells. j-m A375 and SK-Mel-28 cells were treated with 3 μM TM at the indicated times. The expression levels of KLF4 were detected by q-RT-PCR and western blot