Fig. 3

KLF4 inhibited ER stress-induced apoptosis and facilitated cell metastasis in melanoma cells. a-d KLF4 was stably knocked down in Mel-RM and A375 cells and then the cells were treated with 3 μM TM at the indicated times. Cell apoptosis was analysed by western blot and CCK8 assays. Data represent the mean ± SD of three independent experiments. ***p < 0.001 vs. control. e-f Cell migration was detected by a transwell assay. The data represent the means ± SD of three independent experiments; ***p < 0.001 vs. control. g-h KLF4 was stably overexpressed in Me1007 cells and then the cells were treated with 3 μM TM at the indicated times. Cell apoptosis was analysed by western blot and CCK8 assays. Data represent the mean ± SD of three independent experiments. ***p < 0.001 vs. control. i-j Cell migration was detected by a transwell assay. The data represent the means ± SD of three independent experiments; **p < 0.01 vs. control