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Fig. 6 | Journal of Experimental & Clinical Cancer Research

Fig. 6

From: Regulation of the adaptation to ER stress by KLF4 facilitates melanoma cell metastasis via upregulating NUCB2 expression

Fig. 6

KLF4 elevated the adaptation to ER stress and cell metastasis via regulating NUCB2 expression. a-d Mel-RM cells with or without NUCB2 knockdown were treated with 3 μM TM. The cell apoptosis was detected by western blot and CCK-8 assays. Cell migration was analysed using a transwell assay. The data represent the means ± SD of three independent experiments; **p < 0.01 vs. control. e-g Flag-NUCB2 and empty vector were individually transfected into Mel-RM cells with or without KLF4 knockout. The cells were treated with 3 μM TM at the indicated times. Cell apoptosis was detected by a western blot assay and cell migration was analysed using a transwell assay. The data represent the means ± SD of three independent experiments; **p < 0.01 vs. control. h-l KLF4 WT or KO Mel-RM cells with or without NUCB2 expression were subcutaneously injected into nude mice (n = 6 in each group) for tumour formation (1 × 106 cells per mouse, 4 weeks). Representative bright-field imaging of the tumours in the mice implanted the indicated cells. After 4 weeks, mice receiving transplants of indicated cells were sacrificed. The tumour volume and weight were calculated. Caspase-3 activity was measured by a luciferase activity assay; **p < 0.01 vs. control. m-o The cells as indicated (5 × 104) were injected intravenously into nude mice (n = 6 in each group). Representative images of lung and HE pictures were shown (m), and the weight and metastasis nodule of the lung in each group were calculated (g-h); **p < 0.01, ***p < 0.001 vs. control

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