Fig. 5

Effects of Curcumin on HIF-1α activity and IPO7 expression. a Assay of the transcriptional activity of HIF-1α showing that in K526 cells curcumin induced a reduction of HIF-1α activity compared to control cells. The reported values are the mean of three independent experiments. b qPCR (upper panel) and representative Western blot (lower panel) show that curcumin treatment did not affect HIF-1α at both mRNA and protein level. The values (FOI: Fold of Induction) in the histogram are normalized against GAPDH and are the mean ± SD of three independent experiments. c qPCR demonstrates that in K562 cells curcumin induced a decrease of mRNA IPO7 expression. The values (FOI: Fold of Induction) in the histogram are normalized to GAPDH and are the mean ± SD of three independent experiments. d Representative western blot and corresponding densitogram showing that curcumin inhibited the protein expression of IPO7 in K562 cells. In the Western blot assay, actin was used as loading control. Intensities of proteins bands were calculated from the peak area of densitogram by using Image J software. Ctrl: control cells. Statistical significance was calculated vs Ctrl: *p < 0.05, **p < 0.01