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Fig. 6 | Journal of Experimental & Clinical Cancer Research

Fig. 6

From: SWATH-MS based quantitative proteomics analysis reveals that curcumin alters the metabolic enzyme profile of CML cells by affecting the activity of miR-22/IPO7/HIF-1α axis

Fig. 6

Inhibition of IPO7 expression induced by curcumin is miR-22 mediated. a qRT-PCR showing the ability of curcumin to induce in K562 cells a significant increase of miR-22 expression. Ctrl: control. The values (FOI: Fold of Induction) in the histogram are normalized against RNU6–2 and are the mean ± SD of three independent experiments. b qPCR shows that in K562 cells the inhibitory effect on IPO7 mRNA expression induced by miR-22 inhibitor transfection was reverted by curcumin treatment. The values (FOI: Fold of Induction) in the histogram are normalized against GAPDH and are the mean ± SD of three independent experiments. c Representative western blot and corresponding densitogram showing that in K562 cells the inhibitory effect on IPO7 protein expression induced by miR-22 inhibitor transfection was reverted by curcumin treatment. Actin was used as loading control. Intensities of proteins bands were calculated from the peak area of densitogram by using Image J software. d Assay of the transcriptional activity of HIF-1α showing that in K562 cells transfected with miR-22 inhibitor HIF-1α activity was significantly decreased when cells were co-treated with curcumin. Ctrl: control cells. Statistical significance was calculated vs Ctrl (*p < 0.05, **p < 0.01) or as indicated by line (##p < 0.01)

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