Fig. 1

ISL inhibits cell proliferation and induces cell apoptosis in melanoma cells in vitro. a A375/A2058 cells were treated with ISL (0, 10, 20, 40, 80 μM) for 24 h, and cell viability was analyzed by CCK-8 assay. b A375 and A2058 cells were treated with 15 μM and 10 μM ISL respectively, cell proliferation at indicated time (24, 48, 72 h) was measured by CCK-8 assay. c, d Representative images and quantification of colony formation of A375 and A2058 cells after being treated with ISL (0, 5, 10 μM) and ISL (0, 5, 7.5 μM) respectively. e, f Flow cytometry analysis of apoptosis of A375 and A2058 cells after being treated with ISL (0, 7.5, 15 μM) and ISL (0, 5, 10 μM) for 24 h respectively. g, h Expression of apoptosis associated proteins (c-PARP, bcl-2, bax, C-caspase-3) of A375 and A2058 cells after being treated with ISL (0, 7.5, 15 μM) and ISL (0, 5, 10 μM) for 24 h respectively. *P < 0.05, **P < 0.01, ***P < 0.001. n = 3