Fig. 3

MiR-301b reverses the inhibition of ISL in melanoma. A375 and A2058 cells were transfected with miR-301b or control (NC). a, b A375/A2058 cells were treated with ISL (0, 10, 20, 40, 80 μM) for 24 h, and cell viability was analyzed by CCK-8 assay. c, d A375 and A2058 cells were treated with 15 μM and 10 μM ISL respectively, cell proliferation at indicated time (24, 48, 72 h) was measured by CCK-8 assay. e-h Representative images and quantification of colony formation of A375 and A2058 cells after being treated with ISL (0, 15 μM) and ISL (0, 10 μM) respectively. *P < 0.05, **P < 0.01, ***P < 0.001, n = 3