Fig. 7

SP1 is a direct target of miR-324-5p in CC cells. a miR-324-5p and its putative binding sequences in the 3’-UTR of SP1. The mutant binding site was generated in the complementary site for the seed region of miR-324-5p. b miR-324-5p overexpression significantly suppressed, while miR-324-5p loss increased the luciferase activity that carried wild-type (wt) but not mutant (mt) 3’-UTR of SP1. miR-324-5p overexpression reduced the expression of SP1 mRNA (c) and protein (d) in CaSki cells and miR-324-5p knockdown increased the level of SP1 mRNA (c) and protein (d) in C33A cells. e-g The expression of SP1 in miR-324-5p high-expressing tumors was significantly lower than that in miR-324-5p low-expressing tumors, as determined by qRT-PCR (e), immunoblotting (f) and IHC (g). h An inverse correlation between the levels of miR-324-5p and SP1 mRNA was observed in CC tissues. *P < 0.05