Fig. 8

Modulation of SP1 partially abolishes TPT1-AS1 or miR-324-5p-mediated cellular processes in CC. TPT1-AS1 overexpression increased the expression of SP1 mRNA (a) and protein (b) in C33A cells and TPT1-AS1 knockdown reduced the level of SP1 mRNA (a) and protein (b) in CaSki cells. c miR-324-5p-overexpressing or TPT1-AS1-suppressive CaSki cells that were transfected with empty vector (EV) or SP1 overexpression plasmid were subjected to western blot for SP1. miR-324-5p-suppressive or TPT1-AS1-overexpressing C33A cells that were transfected with scrambled siRNA or SP1 siRNA were subjected to western blot for SP1. SP1 restoration abrogated the effects of miR-324-5p overexpression or TPT1-AS1 knockdown on cell colony formation (d), proliferation (e), migration (f), invasion (g) and EMT process (h) of CaSki cells. SP1 knockdown reversed the promotive effects of miR-324-5p knockdown or TPT1-AS1 overexpression in C33A cells (d-h). *P < 0.05