Fig. 1

HEGBC was up-regulated in GBC and associated with poor survival of GBC patients. a The expression of HEGBC in 102 pairs of GBC tissues and adjacent non-tumor tissues was detected using qRT-PCR. P < 0.0001 by Wilcoxon signed-rank test. b The expression of HEGBC in 52 GBC extending beyond the gallbladder (T3 + T4) and in 50 GBC only detected in the gallbladder (T1 + T2). P < 0.0001 by Mann-Whitney test. c The expression of HEGBC in 47 GBC with lymph nodes metastasis (N1 + N2) and in 55 GBC without lymph nodes metastasis (N0). P < 0.0001 by Mann-Whitney test. d The expression of HEGBC in 57 GBC with advanced clinical stages (III-IV) and in 45 GBC with early clinical stages (I-II). P < 0.0001 by Mann-Whitney test. e Kaplan-Meier survival analysis of the correlation between HEGBC expression and cumulative survival of the 102 GBC patients. The median expression level of HEGBC was used as the cut-off. P < 0.0001 by Log-rank test. f Kaplan-Meier survival analysis of the correlation between HEGBC expression and cumulative survival of the 45 stages I-II GBC patients. The median expression level of HEGBC in these 45 GBC was used as the cut-off. P = 0.0173 by Log-rank test. g Kaplan-Meier survival analysis of the correlation between HEGBC expression and cumulative survival of the 57 stages III-IV GBC patients. The median expression level of HEGBC in these 57 GBC was used as the cut-off. P = 0.0023 by Log-rank test. h The expression of HEGBC in human non-tumorigenic biliary epithelial cell line H69 and GBC cell lines SGC-996, NOZ, GBC-SD, and EH-GB2 was detected using qRT-PCR. Results are shown as mean ± s.d. of 3 independent experiments. **P < 0.01, ***P < 0.001 by Student’s t test