Fig. 1

The levels of CpG methylation of the miR-200 family gene promoters in human PDAC. a Quantitative analysis of the CpG methylation of the promoters of miR-200 family members (miR-200a, miR-200b, miR-200c, miR-141, and miR-429). The schematic illustration shows the position of the members of the pre-miR-200 (black bar) and their CpG sites. The start site is indicated by + 1. Each red vertical line indicates an individual CpG site. The location of the CpG sites used for the methylation assays are indicated. b The levels of CpG methylation of the miR-200 family members in 10 paired PDAC and their surrounding non-cancerous tissues on a heatmap. A01-A10 and B01-B10 represent corresponding10 paired human PDAC (A1-A10) and their surrounding non-cancerous tissues (B1-B10) respectively. The colors displayed represent the relative methylation levels in 0.1 increment, from blue = 0 (0% methylated) to red = 1 (100% methylated). c The mean methylation levels of each individual CpG site of miR-200c or miR-141 promoter derived from the 10 paired human PDAC and the surrounding non-cancerous tissues. Error bars are presented as the mean +/− SD (standard deviation). ^* P < 0.05. d The mean methylation levels of the CpG sites of miR-200c or miR-141 promoter of each individual PDAC compared to the paired surrounding non-cancerous tissue. Each circle represents the mean methylation level of the CpG sites of miR-141 or miR-200c. P value as indicated