Fig. 4

Effect of miR-141 and miR-200c on PDAC cell migration, invasion, and metastasis. a-b) miR-141 and miR-200c suppress cell migration; c-d miR-141 and miR-200c suppress cell invasion. PANC-1 and BxPC3 cells were used for the assays. The cells were stained and counted in 10 fields. Magnification (× 200). Data represent the mean +/− SD of three independent experiments. ^** P < 0.01. e-g miR-141 and miR-200c affect the expression of EMT markers. PANC-1 cells were treated with miR-141 or miR-200c and RT-PCR was performed to determine the expression of ZEB-1 (e), Vimentin (f), and E-cadherin (g). h miR-141 and miR-200c inhibited tumor metastasis in the xenograft model. The experiment was performed with five animals in each group, sacrificed 10 weeks after the injection of cancer cells into the spleen of NOD/SCID mice and liver and lungs were harvested. Representative livers and lungs from mice were shown. H&E staining was performed on sections of metastatic tumors and normal liver or lung tissues. Metastatic tumour nodules (T) outlined by a dotted line. Magnification, × 100. i The number of metastatic tumors was quantified by counting the individual tumors on one histological section of the midportion of the liver or lung specimen from each animal. Each group contained five mice