Fig. 5

WIPF1 is a direct target of miR-141 and miR-200c. a miR-141 and miR-200c inhibit the expression of WIPF1 by targeting its 3-UTR. 293 T cells were transfected with lentivirus miR-141 mimics (left panel) or miR-200c mimics (right panel) and wild type (WT) WIPF1 or mutant WIPF1 with 3’-UTR mutations (MUT), together with luciferase reporter. Luciferase activity was assayed 24 h after transfection. Data was normalized against negative control. ^* P < 0.05; ^** P < 0.01; N.S., not significant. b miR-141 and miR-200c inhibit the expression of WIPF1 in the pancreatic cell lines. PANC-1 or BxPC3 cells were infected with lentivirus containing miR-141 or miR-200c (left two panels), or anti-miR-141 or anti-miR-200c (right two panels) and harvested for RT-PCR (upper panels) and Western blotting (lower panels). GAPDH was used as normalization control. ^** P < 0.01. c Inverse correlation between the expression of miR-141/200c and the expression of WIPF1 in 37 human pancreatic cancer samples. The expression of miR-141, miR-200c, and WIPF1 were measured by qRT-PCR and analyzed using the Spearman’s correlation analysis. U6 and GAPDH were used as internal normalization references for miR-141/200c and WIPF1 respectively. d Expression of WIPF1 in 10 paired PDAC and the surrounding non-cancerous tissues by qRT-PCR. GAPDH was used as an internal normalization reference