Fig. 8

miR-141/200c suppresses YAP/TAZ by repressing WIPF1. a miR-141 and miR-200c suppress the expression of YAP/TAZ in PDAC cells. Lentivirus carrying miR-141 or miR-200c was infected into PANC-1 or BxPC-3 cells and the expression of YAP/TAZ was examined by Western Blot. GAPDH was used as reference. b Anti-miR-141 and anti-miR-200c increase the expression of YAP/TAZ in PDAC cells. Experimental design similar as described in (a). c Knockdown of WIPF1 inhibits the expression of YAP/TAZ. PANC-1 or BxPC-3 cells were infected with shCtrl (shControl) or shWIPF1. GAPDH was used as reference. d WIPF1 overcomes the suppressive effect of miR-141/miR-200c on YAP/TAZ expression. PANC-1 or BxPC3 cells were infected with empty vector as control or the lentivirus carrying the entire coding sequence of WIPF1 without the 3’-UTR, and with the lentivirus containing miR-141 or miR-200c. GAPDH was used as reference. e Schematic illustration demonstrating that the methylation-associated silencing of miR-141/200c in human PDAC promotes tumor growth and metastasis by activating the WIPF1-YAP/TAZ pathway, leading to decreased patient survival