Fig. 1

Radiosensitization of PC-3 cells by down-regulation of RelB. a, the endogenous RelB in PC-3 cells was silenced by stably transfecting a RelB siRNA construct. The levels of RelB and its relating proteins were quantified by Western blots. b, the cells were treated with a serial dosage of IR as indicated and cytotoxicity was analyzed by MTT assay. c and d, after IR treatment, proapoptotic and apoptotic cells were detected by flow cytometry (c), and percentage of apoptotic cells was calculated and plotted (d). Mean ± SD was representative of three independent experiments carried out in duplication. *(P < 0.05), **(P < 0.01) show the significances between two groups as indicated