Fig. 3
The effect of HZ08 on ROS production. a, PC-3 cells were treated with HZ08 and IR as indicated. ROS and superoxide anion were quantified using H2DCF-DA (green dye) and MitoSOX (red dye) reagents, respectively. b, DCFDA was used to normalize H2DCF-DA images. c, MitoSOX fluorescence intensity was quantified. To remove the treatment-induced oxidative free radicals, SOD or its heat-inactivated control was added into the cells. Mean ± SD was representative of three independent experiments carried out in duplication. *(P < 0.05), **(P < 0.01) show the significances between two groups as indicated