Fig. 6

The effect of HZ08 on phosphorylation of PI3K, Akt and IKKα. a and b, PC-3 cells were treated with HZ08 alone (a) or treated with HZ08 and IR (b). Cellular extracts from the treated cells were used to quantify the levels of PI3K, p-PI3K, Akt, p-Akt, IKKα, p-IKKα by Western blots. c and d, PC-3 cells were pretreated with LY294002 (25 μM), HZ08 (5 μM) and treated with 6 Gy IR. P-Akt, MnSOD in cellular extracts and RelB in nuclear extracts were quantified by Western blots (c). Cytotoxicity was analyzed using MTT by three independent experiments and plotted as mean ± SD. ** (P < 0.01) shows the significances between two groups as indicated (d)