Fig. 2
From: ANXA2 promotes esophageal cancer progression by activating MYC-HIF1A-VEGF axis
ANXA2 potentiates cell invasion and migration by activating HIF1A-VEGF signaling in ESCC cells. a-d KYSE30 and KYSE150 cells were transfected with ANXA2 siRNAs or control non-silencing siRNA. VEGF and HIF1A mRNA levels were analyzed using real-time RT-PCR (a and c), and VEGF and HIF1A protein levels were determined by western blotting (b and d). e-f Transwell assays were used to analyze cell migration and invasion ability. e ESCC cells were transfected with HIF1A siRNAs or non-silencing siRNA. f ESCC cells stably expressing ANXA2-shRNA were transfected with pcDNA3.1-HIF1A or empty vector. Representative results (left) and statistical plots (right) are shown. **, P < 0.01; ***, P < 0.001