Fig. 2

Upregulation of RBM38 increases expression of wtp53 and inhibition expression of mdm2. a The fold changes of mdm2, wtp53, RBM38 are shown below each lane. The intensity of the bands were determined using densitometric analysis. Data are representative from three independent experiments. b The luciferase activity for the reporter carrying mdm2 3’-UTR-A, -B or -C is repressed by RBM38. SMMC7721-RBM38-OE, SMMC7721-RBM38-NC, HepG2-RBM38-OE, HepG2-RBM38-NC cells were transfected with pGL3 reporter carrying various regions of mdm2 3’-UTR. Cells were then harvested for luciferase assay. The fold change in relative luciferase activity in RBM38-OE cells were compared to NC cells