Fig. 3

MIR31HG was a target of miR-575. a qRT-PCR analysis for MIR31HG regulated miR-575 expressions in SMMC7721 and HepG2 cells (**P < 0.01 versus pcDNA3.1 group; ##P < 0.01 versus sh-NC group). b qRT-PCR analysis for miR-575 impaired MIR31HG expressions in SMMC7721 and HepG2 cells (**P < 0.01 versus pre-NC group; ##P < 0.01 versus anti-NC group). c The predicted miR-575 binding sites in MIR31HG (MIR31HG-WT) or and the designed mutant sequence (MIR31HG-MUT) were indicated. d Luciferase reporter assay of 293 T and SMMC7721 cells co-transfected with MIR31HG-WT or MIR31HG-MUT and pre-NC or pre-miR-384 (**P < 0.01 versus MIR31HG-WT + pre-NC group). e, f, g and h RIP assay was performed using input from cell lysate, normal mouse IgG or anti-Ago2. Relative expression levels of MIR31HG and miR-575 were detected by qRT-PCR (*P < 0.05, **P < 0.01 versus anti-normal IgG group of respective group, #P < 0.05, ##P < 0.01 versus anti-Ago2 in control group)