Fig. 4

MiR-575 was upregulated in HCC cell lines and tissues, and acted as an oncogene in HCC. a qRT-PCR analysis of miR-575 expressions in L02 cells and four cell lines (**P < 0.01). b qRT-PCR analysis of miR-575 expressions in 42 pairs of HCC tissues and adjacent non-tumor tissues (P < 0.01). c The expression of MIR31HG was negatively correlated with that of miR-575 (r = − 0.6123, P < 0.01) in clinical specimens. d Growth curves of SMMC7721 and HepG2 cells after transfection with pre-miR-575 or anti-miR-575 were determined by MTS assays. e Clone formation assay of differently treated HCC cells. f The healing abilities of miR-575 in SMMC7721 and HepG2 cells were determined with Wound healing assay (magnification, 50 ×; scale bar, 500 μm). g and h Quantification number of migration and invasion cells with different expression levels of miR-575 (magnification, 200 ×; scale bar, 125 μm). (*P < 0.05, **P < 0.01 versus pre-NC group; #P < 0.05, ##P < 0.01 versus anti-NC group)