Fig. 6

MiR-575 mediated the effects of MIR31HG on cell proliferation and metastasis. a MTS assay was applied to evaluate the proliferation effect of MIR31HG and miR-575 on SMMC7721 and HepG2 cells. b Clone formation assay of SMMC7721 and HepG2 cells with the expression of MIR31HG and miR-575 changed. c Wound healing assay was used to evaluate the migratory ability of MIR31HG and miR-575 on SMMC7721 and HepG2 cells (magnification, 50 ×; scale bar, 500 μm). d and e Quantification of migration and invasion cells with the expression of MIR31HG and miR-575 changed (magnification, 200 ×; scale bar, 125 μm). f and g qRT-PCR and Western blot analysis for MIR31HG and miR-575 regulating the expression of ST7L. (**P < 0.01 versus pcDNA3.1-MIR31HG+ pre-miR-575 group; ##P < 0.01 versus sh-MIR31HG + anti-miR-575 group; ▲▲P < 0.01 versus control group (pcDNA3.1 + pre-NC group))