Fig. 5From: RIF1 promotes human epithelial ovarian cancer growth and progression via activating human telomerase reverse transcriptase expressionRIF1 regulated hTERT expression and promoter activity in EOC cells by binding to the promoter of hTERT. a and b hTERT mRNA expression was down-regulated in RIF1 knockdown and up-regulated in RIF1 overexpressed OVCAR3 and A2780 cells. c RIF1 regulated the protein expression of hTERT and the hTERT downstream PI3K/AKT signaling proteins in OVCAR3 and A2780 cells. d Relative hTERT promoter activity in RIF1 knockdown and RIF1 overexpressed OVCAR3 and A2780 cells. e Schematic diagram of five pairs of primers designed for ChIP spanning the hTERT promoter. f RIF1 binds to the hTERT promoter indicated by ChIP in OVCAR3 and A2780 cells. IgG from rabbit served as a control. g A schematic diagram of RIF1 wild type and mutant binding sites in hTERT promoter. h Dual-luciferase reporter gene assay was conducted in OVCAR3 and A2780 cells with overexpression of RIF1. Data are expressed as means ± SD of three independent experiments. * P < 0.05, ** P < 0.01, *** P < 0.001Back to article page