Fig. 7

Overexpression of hTERT reversed the inhibition of cell viability, colony formation sphere formation mediated by RIF1 knockdown in EOC cells. a Efficiency of hTERT overexpression (hTERT over) in OVCAR3 and A2780 cells was verified by real-time qPCR and western blot. b Cell viability at 72 h was examined by MTS assay. c and d Colony formation assay of scrambled control or RIF1 knockdown OVCAR3 and A2780 cells with or without hTERT overexpression. e and f Representative images and quantification of spheres formed by scrambled control or RIF1 knockdown OVCAR3 and A2780 cells with or without hTERT overexpression. Scale bar, 100 μm. The results were shown as means ± SD of three independent experiments. * P < 0.05, ** P < 0.01, *** P < 0.001