Fig. 5

MKRN2 mediates PI3Kp85α ubiquitination and degradation to affect the Akt pathway. a, b mRNA levels of the p85α subunit of PI3K in a A549 and b H1299 cells transfected with si-MKRN2. c Relative PI3Kp85α protein expression (normalized to GAPDH) as measured by densitometry. *P < 0.05; **P < 0.01. d, e Effects of MKRN2 expression on PI3Kp85α ubiquitination. d A549 and e H1299 cells transfected with si-MKRN2 or the MKRN2-expression plasmid along with HA-ubiquitin (Ub). Levels of PI3Kp85α ubiquitination were evaluated by immunoprecipitation using the anti-PI3Kp85α antibody, followed by anti-HA immunoblotting