Table 1 List of antibodies used for western blot
Antibody name | Source | Catalog number | Host | Dilution |
|---|---|---|---|---|
MKRN2 | Sigma-Aldrich | HPA037559 | Rabbit | 1:100 |
GAPDH | Beyotime | AF0006 | Mouse | 1:1000 |
RhoA | Cell Signaling Technology | 2117 | Rabbit | 1:500 |
MMP9 | Cell Signaling Technology | 13,667 | Rabbit | 1:500 |
ROCK1 | Wanlei Bio. | Wl01761 | Rabbit | 1:500 |
PI3Kp85α | Santa Cruz Biotechnology | SC-423 | Rabbit | 1:100 |
PI3Kp85α | Cell Signaling Technology | 4257 | Rabbit | 1:500 |
PI3Kp85α | Cell Signaling Technology | 13,666 | Mouse | 1:50 |
AKT | Cell Signaling Technology | 4685 | Rabbit | 1:1000 |
p-AKT(ser473) | Cell Signaling Technology | 4060 | Rabbit | 1:500 |
IgG | Byotime | A7028 | Mouse | 1:50 |
HA | TransGen Biotech | HT301 | Mouse | 1:1000 |