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Fig. 4 | Journal of Experimental & Clinical Cancer Research

Fig. 4

From: TSSC3 promotes autophagy via inactivating the Src-mediated PI3K/Akt/mTOR pathway to suppress tumorigenesis and metastasis in osteosarcoma, and predicts a favorable prognosis

Fig. 4

Crucial involvement of autophagy in TSSC3-mediated tumorigenesis in osteosarcoma cells in vivo. a Representative xenograft tumors of sacrificed nude mice at the end of the experiment with treatments as indicated. b Growth curves of subcutaneous xenograft tumors show that lenti-overTSSC3 transfected MTF cells displayed a reduced ability to form tumors in nude mice, while their combination with lenti-shATG5 blocked the ability of TSSC3 to inhibit the tumorigenesis of osteosarcoma in vivo. *P < 0.05. c Representative electron microscopy images showing that TSSC3-overexpression induces autophagy and ATG5 knockdown blocked TSSC3-induced autophagy in vivo. The numbers of autophagic vesicles (AVs) in each cell was quantified in 20 randomly selected cells per group. * P < 0.05, Scale bars: 1 μm. d Confirmation of the effect of TSSC3-overexpression and ATG5 knockdown on autophagy in osteosarcoma cells in vivo using autophagy-related proteins immunohistochemical staining. The quantitation of IHC scores is shown in Additional file 2: Figure S4). Scale bars: 100 μm. e Quantification of Ki67 staining in xenografts from MTF cells treated as indicated. Representative images are shown in the Additional file 2: Figure S4). *P < 0.05

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