Fig. 1

Protein kinase inhibitor screen for reprogramming GBM cells. a & b Screening design and primary screening results. Human GBM cells (U118) expressing MAP2-promoter-RFP were seeded in 96-well plates. Kinase inhibitors were added at a final concentration of 2 μM in the induction medium. The medium was changed every other day until day 7 when cells converted to RFP-positive cells. Images were taken on day 7 after inhibitor treatment. Positive induced neural-like cells were counted by RFP-positive staining and quantified on day 7. The histogram represents the ratio of normalized data for kinase inhibitor treatment as compared with DMSO. c Secondary screening results. U118 cells were treated with candidate kinase inhibitors. R + Y: Rapamycin+Y27632; P + Y: P529 + Y27632. Quantitative data are the mean ± SEM from three independent experiments