Fig. 5

Proliferation and invasion ability of iNs. a GBM GM97 and iN (3 weeks induction) cells were treated with 2 μM P + Y. Proliferating cells (viable) were evaluated by trypan blue dye exclusion assay at day 4 after treatment. Shown are the mean fold change in cell number relative to the 24-h time point and SEM from three treatments. b TUNEL assay was performed for GBM GM97 and iN cells treated with 2 μM P + Y. Apoptotic cells were evaluated at day 4 after treatment from three treatments. Etoposide treatment (5 μM) was used as a positive control. c-e GBM GM97 and iN cells were seeded for soft agar assays (c & d) and invasion assays (e). Quantitative data are the mean ± SEM from three independent experiments