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Fig. 6 | Journal of Experimental & Clinical Cancer Research

Fig. 6

From: Reprogramming glioblastoma multiforme cells into neurons by protein kinase inhibitors

Fig. 6

Reprogramming orthotopic GBMs in mice. a Experimental design for tumor formation in mice. b Parental GBM cells (Control) and iNs were implanted into the same nude mice (left and right side each mouse, respectively) and the tumor formation was measured. c & d GBM tumors generated by GM97 cells in nude mice with postoperative recurrence model. ROCK-mTOR inhibitors (P529 and Y27632, P + Y) with induction media in the MDD pellet were administered directly into the resection cavity of GBMs. P + Y without induction medium, medium only (both in the MDD pellets) or no treatment were used as controls. Tumor recurrence (c) and mouse survival (d) were measured (n = 10). e Postoperative residual GFP-GBM were treated with ROCK-mTOR inhibitors (P + Y) with induction medium. GFP-positive cells expressed the mature neuronal markers MAP2 and Synapsin 2 weeks after transplantation. Data are presented as mean ± SEM from 5 mice (300 cells/section and 2 sections per mouse were counted). The scale bar represents 10 μm. f-g Ki67 (IHC) and apoptotic (TUNEL assay) cells were evaluated in postoperative brain tissues treated with P + Y and induction media. Normal brain tissue was as the control. Ki67 and apoptotic cells from recurred tumors treated with P + Y without induction media, media only or no treatment were also examined. Scale bar 10 μm

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