Fig. 4

PAR1 internalization can promote Twist1 nuclear translocation. a Confocal imaging of PAR1 and Twist1 in PLC-PRF-5 cells transfected with PAR1 vectors. b Knockdown of PAR1 decreased Twist1 nuclear translocation in HepG2/M cells. c PAR1 internalization can occur after thrombin binding, as shown in the confocal microscopy images. Twist1 translocation to the nucleus was also increased. Mander’s overlap coefficient was used to quantify the degree of colocalization of both proteins (mean ± sd; n = 3 in triplicate; *P < 0.05, **P < 0.01)